. The working pump and the equilibrating pump Every Have got a piston whose back and forth motion maintains a continuing flow rate of around many mL/min and presents the high output strain needed to push the cellular stage through the chromatographic column.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.
Rotating the inner valve (proven in red) towards the inject posture directs the cellular phase from the sample loop and onto the column.
-hydroxybenzoic acid elutes extra slowly and gradually. Although we can resolve thoroughly these two solutes using cellular stage that is definitely 16% v/v acetonitrile, we are unable to take care of them Should the cell phase is ten% tetrahydrofuran.
Peak places: The realm beneath each peak in the chromatogram is proportional to the quantity of analyte current, permitting for quantification.
ⅱ. 액체 크로마토그래피 정보에 대해 더 자세한 내용은 크로마토그래피 학습센터를 참고해주세요.
The get more info force makes the technique much faster as compared to column chromatography. This permits using Significantly more compact particles for your column packing content.
Lots of differing types of detectors are actually use to observe HPLC separations, nearly all of which use the spectroscopic strategies from Chapter 10 or perhaps the electrochemical strategies from Chapter eleven.
Ion-Trade chromatography is predicated around the separation of substances based on their demand. The stationary stage contains charged groups that appeal to and keep oppositely billed ions from the sample.
*본 포스팅의 저작권은 써모 피셔 사이언티픽에 있으며, 콘텐츠의 무단 복제 및 수정, 재배포를 get more info 금지합니다.
Soon after positioning the sample inside the sample reservoir the injection system is totally automated. The injector injects the sample in the continuously flowing mobile section stream that carries the sample towards the HPLC column.
The Show are going to be recorded for a number of peaks- each one represents the Each and every ingredient within the combination which could soak up UV gentle. The region of the height is proportional to the amount of the ingredient handed in the detector.
The injector is positioned following the pump to introduce the sample into your mobile stage. Syringes are the most usual sample injectors. Inside the car-injector, injection with the sample takes place automatically in the predetermined time.